lysis buffer Search Results


lysis buffer  (TaKaRa)
97
TaKaRa lysis buffer
Lysis Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna rna lysis buffer  (Zymo Research)
95
Zymo Research dna rna lysis buffer
Dna Rna Lysis Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lysis buffer 10x  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc cell lysis buffer 10x
Cell Lysis Buffer 10x, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lysis buffer a  (Bio-Rad)
97
Bio-Rad lysis buffer a
Lysis Buffer A, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ripa buffer  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology ripa buffer
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Ripa Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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triton x 100  (Thermo Fisher)
99
Thermo Fisher triton x 100
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Triton X 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high salt lysis buffer  (Thermo Fisher)
99
Thermo Fisher high salt lysis buffer
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
High Salt Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lysis  (Valiant Co Ltd)
95
Valiant Co Ltd cell lysis
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Cell Lysis, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ice cold ripa buffer  (Boston BioProducts)
98
Boston BioProducts ice cold ripa buffer
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Ice Cold Ripa Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna lysis buffer  (Zymo Research)
98
Zymo Research rna lysis buffer
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Rna Lysis Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs  (Boster Bio)
96
Boster Bio pbs
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Pbs, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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radio immunoprecipitation assay ripa lysis buffer  (MedChemExpress)
97
MedChemExpress radio immunoprecipitation assay ripa lysis buffer
FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in <t>RIPA</t> buffer <t>was</t> <t>immunoprecipitated</t> using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.
Radio Immunoprecipitation Assay Ripa Lysis Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in RIPA buffer was immunoprecipitated using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.

Journal: Virology

Article Title: The role of v-Fgr myristoylation and the Gag domain in membrane binding and cellular transformation.

doi: 10.1006/viro.1998.9323

Figure Lengend Snippet: FIG. 8. Expression of v-Fgr proteins in NIH-3T3 cells. (A) NIH-3T3 cells transfected with wild-type (wt) and indicated mutant pMV7-vfgr constructs were selected in the presence of G418. Whole cell lysate (1 mg) in kinase buffer was subjected to immunoprecipitation analysis using a Fgr-specific antibody. Immunoprecipitates were washed three times in kinase buffer and once in kinase reaction buffer (see Materials and Methods). In vitro kinase reactions were performed at 30°C for 20 min in 40 ml kinase reaction buffer supplemented with 100 mM Na2VO4, 20 mM ATP, and 5 mCi [32P]g ATP. Proteins were resolved using 10% SDS–PAGE, and the resulting gel was subjected to autoradiography. Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in RIPA buffer was immunoprecipitated using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods). Fgr protein are indicated by the arrows.

Article Snippet: Autophosphorylated proteins are indicated by the arrows. (B) Whole cell lysate (1 mg) in RIPA buffer was immunoprecipitated using a Fgr specific antiserum (Santa Cruz), resolved by SDS–PAGE, and subjected to Western blot analysis using the same antiserum (see Materials and Methods).

Techniques: Expressing, Transfection, Mutagenesis, Construct, Immunoprecipitation, In Vitro, SDS Page, Autoradiography, Western Blot